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Expression of Recombinant Proteins with Uniform N-Termini
Heterologously expressed proteins in Escherichia coli may undergo unwanted N-terminal processing by methionine and proline aminopeptidases. To overcome this problem, we present a system where the gene of interest is cloned as a fusion to a self-splicing mini-intein. Furthermore, this fusion construc...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2011
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3107599/ https://ncbi.nlm.nih.gov/pubmed/21125386 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61737-967-3_10 |
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