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Sequencing Multimegabase-Template DNA with BigDye Terminator Chemistry

Using the recently introduced BigDye™ terminators, large-template DNA can be directly sequenced with custom primers on automated instruments. Cycle sequencing conditions are presented to sequence DNA samples isolated from a number of microbial genomes including 750-kb Ureaplasma urealyticum, 1.2-Mb...

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Published in:Genome Res
Main Authors: Heiner, Cheryl R., Hunkapiller, Kathryn L., Chen, Shiaw-Min, Glass, John I., Chen, Ellson Y.
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 1998
Subjects:
Genome Methods
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310720/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9582199/
https://ncbi.nlm.nih.govhttps://doi.org/10.1101/gr.8.5.557
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