Cloning, expression, purification, crystallization and preliminary X-ray crystallo­graphic study of GK0767, the copper-containing nitrite reductase from Geobacillus kaustophilus

The soluble region (residues 32–354) of GK0767, a copper-containing nitrite reductase from the thermophilic Gram-positive bacterium Geobacillus kaustophilus HTA426, has been cloned and overexpressed in Escherichia coli. The purified recombinant protein was crystallized using the hanging-drop vapour-...

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Main Authors: Fukuda, Yohta, Tamada, Taro, Takami, Hideto, Suzuki, Shinnichiro, Inoue, Tsuyoshi, Nojiri, Masaki
Formato: Artigo
Idioma:Inglês
Publicado: International Union of Crystallography 2011
Assuntos:
Crystallization Communications
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3107145/
https://ncbi.nlm.nih.gov/pubmed/21636914
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111013297
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