A PCR method for introducing mutations into cloned DNA by joining an internal primer to a tagged flanking primer.

Ítems similars

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• PrecisePrimer: an easy-to-use web server for designing PCR primers for DNA library cloning and DNA shuffling
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• Excision of DNA segments introduced into cloning vectors by the poly(dA-dT) joining method.
  per: Goff, S P, et al.
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• Fusion primer and nested integrated PCR (FPNI-PCR): a new high-efficiency strategy for rapid chromosome walking or flanking sequence cloning
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