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Sequence specific generation of a DNA panhandle permits PCR amplification of unknown flanking DNA.
We present a novel method for the PCR amplification of unknown DNA that flanks a known segment directly from human genomic DNA. PCR requires that primer annealing sites be present on each end of the DNA segment that is to be amplified. In this method, known DNA is placed on the uncharacterized side...
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| Główni autorzy: | , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
1992
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| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC310428/ https://ncbi.nlm.nih.gov/pubmed/1371352 |
| Etykiety: |
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