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Highly efficient generation of recombinant baculoviruses by enzymatically medicated site-specific in vitro recombination.

We have used the Cre-lox system of bacteriophage P1 to develop a highly efficient in vitrosystem for construction of recombinant baculoviruses. A positive visual selection has been included to make identification of recombinant viral progeny rapid and straightforward. We report recombination frequen...

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Publicat a:Nucleic Acids Res
Autors principals: Peakman, T C, Harris, R A, Gewert, D R
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1992
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC310413/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1741284/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/20.3.495
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