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Phasor-based single-molecule fluorescence lifetime imaging using a wide-field photon-counting detector

Fluorescence lifetime imaging (FLIM) is a powerful approach to studying the immediate environment of molecules. For example, it is used in biology to study changes in the chemical environment, or to study binding processes, aggregation, and conformational changes by measuring Förster resonance energ...

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Bibliographic Details
Main Authors: Colyer, R., Siegmund, O., Tremsin, A., Vallerga, J., Weiss, S., Michalet, X.
Format: Artigo
Language:Inglês
Published: 2009
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3103255/
https://ncbi.nlm.nih.gov/pubmed/21625298
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1117/12.809496
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