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Real time kinetics of restriction endonuclease cleavage monitored by fluorescence resonance energy transfer.

The kinetics of PaeR7 endonuclease-catalysed cleavage reactions of fluorophor-labeled oligonucleotide substrates have been examined using fluorescence resonance energy transfer (FRET). A series of duplex substrates were synthesized with an internal CTCGAG PaeR7 recognition site and donor (fluorescei...

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Main Authors: Ghosh, S S, Eis, P S, Blumeyer, K, Fearon, K, Millar, D P
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1994
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC310290/
https://ncbi.nlm.nih.gov/pubmed/8065930
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