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1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer

BACKGROUND: Quantitative real-time PCR (qPCR) is becoming increasingly important for DNA genotyping and gene expression analysis. For continuous monitoring of the production of PCR amplicons DNA-intercalating dyes are widely used. Recently, we have introduced a new qPCR mix which showed improved amp...

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Bibliografiske detaljer
Main Authors: Horáková, Helena, Polakovičová, Iva, Shaik, Gouse M, Eitler, Jiří, Bugajev, Viktor, Dráberová, Lubica, Dráber, Petr
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3102612/
https://ncbi.nlm.nih.gov/pubmed/21501492
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-11-41
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