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Rapid and Enhanced Proteolytic Digestion using Electric-Field-oriented Enzyme Reactor
We have created a novel enzyme reactor using electric field-mediated orientation and immobilization of proteolytic enzymes (trypsin/chymotrypsin) on biocompatible PVDF membranes in a continuous flow-through chamber. Using less than 5 minutes, this reactor in various enzyme combinations can produce e...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3102135/ https://ncbi.nlm.nih.gov/pubmed/21338726 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jprot.2011.02.007 |
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