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Digital Isothermal Quantification of Nucleic Acids via Simultaneous Chemical Initiation of Recombinase Polymerase Amplification Reactions on SlipChip

In this paper, digital quantitative detection of nucleic acids was achieved at the single-molecule level by chemical initiation of over one thousand sequence-specific, nanoliter, isothermal amplification reactions in parallel. Digital polymerase chain reaction (digital PCR), a method used for quanti...

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Bibliografische gegevens
Hoofdauteurs: Shen, Feng, Davydova, Elena K., Du, Wenbin, Kreutz, Jason E., Piepenburg, Olaf, Ismagilov, Rustem F.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2011
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3101872/
https://ncbi.nlm.nih.gov/pubmed/21476587
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac200247e
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