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Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.

Trinucleotide phosphoramidites representing codons for all 20 amino acids have been prepared and used in automated, solid-phase DNA synthesis. In contrast to an earlier report, we show that these substances can be used to introduce entire codons into oligonucleotides in excess of 98% yield, and are...

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Detalles Bibliográficos
Main Authors: Virnekäs, B, Ge, L, Plückthun, A, Schneider, K C, Wellnhofer, G, Moroney, S E
Formato: Artigo
Idioma:Inglês
Publicado: 1994
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC310122/
https://ncbi.nlm.nih.gov/pubmed/7838712
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