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'Cold SSCP': a simple, rapid and non-radioactive method for optimized single-strand conformation polymorphism analyses.

A rapid (< 2.5 hrs) method for single-strand conformation polymorphism (SSCP) analysis of PCR products that allows the use of ethidium bromide staining is described. PCR products ranging in size from 117 to 256 bp were evaluated for point mutations and polymorphisms by 'cold SSCP' in co...

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Bibliographic Details
Published in:Nucleic Acids Res
Main Authors: Hongyo, T, Buzard, G S, Calvert, R J, Weghorst, C M
Format: Artigo
Language:Inglês
Published: Oxford University Press 1993
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309858/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8367279/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.16.3637
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