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Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimization.
Differential display has been developed as a tool to detect and characterize altered gene expression in eukaryotic cells. The basic principle is to systematically amplify messenger RNAs and then distribute their 3' termini on a denaturing polyacrylamide gel. Here we provide methodological detai...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1993
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC309766/ https://ncbi.nlm.nih.gov/pubmed/8341601 |
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