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A method to identify and characterize Z-DNA binding proteins using a linear oligodeoxynucleotide.
An oligodeoxynucleotide that readily flips to the Z-DNA conformation in 10mM MgCl2 was produced by using Klenow enzyme to incorporate 5-bromodeoxycytosine and deoxyguanosine into a (dC-dG)22 template. During synthesis the oligomer can be labeled with 32P to high specific activity. The labeled oligod...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
1993
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| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC309597/ https://ncbi.nlm.nih.gov/pubmed/8332463 |
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