A PCR-based method for high stringency screening of DNA libraries.
A rapid method for cloning genomic DNA utilizing a PCR-based screening protocol is described. A murine genomic library in lambda phage was subdivided into 64 wells, each containing 1000 clones, and propagated in bacteria. Amplified phage from each of 8 wells across columns, and each of 8 wells down...
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| Izdano u: | Nucleic Acids Res |
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| Glavni autor: | |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1993
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| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC309591/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8332459/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.11.2627 |
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