Laser microdissection of conifer stem tissues: Isolation and analysis of high quality RNA, terpene synthase enzyme activity and terpenoid metabolites from resin ducts and cambial zone tissue of white spruce (Picea glauca)

BACKGROUND: Laser microdissection (LMD) has been established for isolation of individual tissue types from herbaceous plants. However, there are few reports of cell- and tissue-specific analysis in woody perennials. While microdissected tissues are commonly analyzed for gene expression, reports of p...

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Détails bibliographiques
Auteurs principaux: Abbott, Eric, Hall, Dawn, Hamberger, Björn, Bohlmann, Jörg
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2010
Sujets:
Methodology Article
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3095273/
https://ncbi.nlm.nih.gov/pubmed/20540781
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2229-10-106
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