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One for All—A Highly Efficient and Versatile Method for Fluorescent Immunostaining in Fish Embryos

BACKGROUND: For the detection and sub-cellular (co)-localization of proteins in the context of the tissue or organism immunostaining in whole mount preparations or on sections is still the best approach. So far, each antibody required its own fixation and antigen retrieval protocol so that optimizin...

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Autors principals: Inoue, Daigo, Wittbrodt, Joachim
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3094454/
https://ncbi.nlm.nih.gov/pubmed/21603650
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0019713
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