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The biochemical defects of prp4-1 and prp6-1 yeast splicing mutants reveal that the PRP6 protein is required for the accumulation of the [U4/U6.U5] tri-snRNP.

We have raised specific antibodies against the PRP6 protein and shown that the U4, U5 and U6 snRNAs are co-precipitated with this protein. Using splicing extracts prepared from in vivo heat-inactivated cells, we have characterized the prp4-1 and prp6-1 biochemical defects. In inactivated prp4-1 cell...

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Bibliografiske detaljer
Main Authors: Galisson, F, Legrain, P
Format: Artigo
Sprog:Inglês
Udgivet: 1993
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC309362/
https://ncbi.nlm.nih.gov/pubmed/8479905
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