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Fluorescence-based fixative and vital staining of lipid droplets in Caenorhabditis elegans reveal fat stores using microscopy and flow cytometry approaches

The proportions of body fat and fat-free mass are determining factors of adiposity-associated diseases. Work in Caenorhabditis elegans has revealed evolutionarily conserved pathways of fat metabolism. Nevertheless, analysis of body composition and fat distribution in the nematodes has only been part...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Klapper, Maja, Ehmke, Madeleine, Palgunow, Daniela, Böhme, Mike, Matthäus, Christian, Bergner, Gero, Dietzek, Benjamin, Popp, Jürgen, Döring, Frank
Format: Artigo
Sprache:Inglês
Veröffentlicht: The American Society for Biochemistry and Molecular Biology 2011
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3090249/
https://ncbi.nlm.nih.gov/pubmed/21421847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D011940
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