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A Simple and Robust Method for Determining the Number of Basic Sites in Peptides and Proteins using Electrospray Ionization Mass Spectrometry

A solution additive has been discovered that can be used to measure the number of basic sites in a peptide or protein using electrospray ionization (ESI) mass spectrometry. Addition of millimolar amounts of perchloric acid (HClO(4)) to aqueous solutions that contain peptides or proteins results in t...

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Détails bibliographiques
Auteurs principaux: Flick, Tawnya G., Merenbloom, Samuel I., Evan, R. Williams
Format: Artigo
Langue:Inglês
Publié: 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3089436/
https://ncbi.nlm.nih.gov/pubmed/21338067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac1031012
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