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A Simple and Robust Method for Determining the Number of Basic Sites in Peptides and Proteins using Electrospray Ionization Mass Spectrometry
A solution additive has been discovered that can be used to measure the number of basic sites in a peptide or protein using electrospray ionization (ESI) mass spectrometry. Addition of millimolar amounts of perchloric acid (HClO(4)) to aqueous solutions that contain peptides or proteins results in t...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3089436/ https://ncbi.nlm.nih.gov/pubmed/21338067 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac1031012 |
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