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Crystallization and preliminary X-ray diffraction studies of the precursor protein of a thermostable variant of papain

The crystallization of a recombinant thermostable variant of pro-papain has been carried out. The mutant pro-enzyme was expressed in Escherichia coli as inclusion bodies, refolded, purified and crystallized. The crystals belonged to space group P2(1), with unit-cell parameters a = 42.9, b = 74.8, c...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Roy, Sumana, Choudhury, Debi, Chakrabarti, Chandana, Biswas, Sampa, Dattagupta, J. K.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: International Union of Crystallography 2011
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3087658/
https://ncbi.nlm.nih.gov/pubmed/21543879
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111010888
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