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A microfluidic device with fluorimetric detection for intracellular components analysis

An integrated microfluidic system that coupled lysis of two cell lines: L929 fibroblasts and A549 epithelial cells, with fluorescence-based enzyme assay was developed to determine β-glucocerebrosidase activity. The microdevice fabricated in poly(dimethylsiloxane) consists of three main parts: a chem...

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Detalhes bibliográficos
Main Authors: Kwapiszewski, Radosław, Skolimowski, Maciej, Ziółkowska, Karina, Jędrych, Elżbieta, Chudy, Michał, Dybko, Artur, Brzózka, Zbigniew
Formato: Artigo
Idioma:Inglês
Publicado em: Springer US 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3085115/
https://ncbi.nlm.nih.gov/pubmed/21222164
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10544-011-9511-0
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