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Stable Isotope Labeling Strategy for Protein–Ligand Binding Analysis in Multi-Component Protein Mixtures

Described here is a stable isotope labeling protocol that can be used with a chemical modification- and mass spectrometry-based protein–ligand binding assay for detecting and quantifying both the direct and indirect binding events that result from protein–ligand binding interactions. The protocol ut...

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Detalhes bibliográficos
Main Authors: DeArmond, Patrick D., West, Graham M., Huang, Hai-Tsang, Fitzgerald, Michael C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3085011/
https://ncbi.nlm.nih.gov/pubmed/21472561
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13361-010-0060-1
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