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Internal fluorescence labeling with fluorescent deoxynucleotides in two-label peak-height encoded DNA sequencing by capillary electrophoresis.

Fluorescently labeled deoxynucleotides were used for internal labeling of DNA sequencing fragments generated in a two-color peak-height encoded protocol. Sequenase and a manganese-containing buffer were used to generate uniform peak heights. Tetramethyl rhodamine - dATP was used in a labeling step,...

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Publicat a:Nucleic Acids Res
Autors principals: Starke, H R, Yan, J Y, Zhang, J Z, Mühlegger, K, Effgen, K, Dovichi, N J
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 1994
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC308401/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7937123/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.19.3997
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