Direct selection of binding proficient/catalytic deficient variants of BamHI endonuclease.
Variants of BamHI endonuclease in which the glutamate 113 residue has been changed to lysine or the aspartate 94 to asparagine were shown to behave as repressor molecules in vivo. This was demonstrated by placing a BamHI recognition sequence, GGATCC, positioned as an operator sequence in an antisens...
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| Published in: | Nucleic Acids Res |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
1994
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| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307931/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7908739/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/22.6.1068 |
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