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Solubilization of a Membrane Protein by Combinatorial Supercharging

Hydrophobic and aggregation-prone, membrane proteins often prove too insoluble for conventional in vitro biochemical studies. To engineer soluble variants of human caveolin-1, a phage-displayed library of caveolin variants targeted the hydrophobic intra-membrane domain with substitutions to charged...

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Библиографические подробности
Главные авторы: Hajduczki, Agnes, Majumdar, Sudipta, Fricke, Marie, Brown, Isola A.M., Weiss, Gregory A.
Формат: Artigo
Язык:Inglês
Опубликовано: 2011
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3078193/
https://ncbi.nlm.nih.gov/pubmed/21192634
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/cb1001729
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