Radiation damage in protein crystals is reduced with a micron-sized X-ray beam

Radiation damage is a major limitation in crystallography of biological macromolecules, even for cryocooled samples, and is particularly acute in microdiffraction. For the X-ray energies most commonly used for protein crystallography at synchrotron sources, photoelectrons are the predominant source...

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Main Authors: Sanishvili, Ruslan, Yoder, Derek W., Pothineni, Sudhir Babu, Rosenbaum, Gerd, Xu, Shenglan, Vogt, Stefan, Stepanov, Sergey, Makarov, Oleg A., Corcoran, Stephen, Benn, Richard, Nagarajan, Venugopalan, Smith, Janet L., Fischetti, Robert F.
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 2011
Fag:
Biological Sciences
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3076849/
https://ncbi.nlm.nih.gov/pubmed/21444772
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1017701108
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