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Structural studies of the mechanism for biosensing antibiotics in a fluorescein-labeled β-lactamase

BACKGROUND: β-lactamase conjugated with environment-sensitive fluorescein molecule to residue 166 on the Ω-loop near its catalytic site is a highly effective biosensor for β-lactam antibiotics. Yet the molecular mechanism of such fluorescence-based biosensing is not well understood. RESULTS: Here we...

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Autors principals: Wong, Wai-Ting, Au, Ho-Wah, Yap, Hong-Kin, Leung, Yun-Chung, Wong, Kwok-Yin, Zhao, Yanxiang
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3076226/
https://ncbi.nlm.nih.gov/pubmed/21443768
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6807-11-15
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