A new vector for recombination-based cloning of large DNA fragments from yeast artificial chromosomes.
The functional analysis of genes frequently requires manipulation of large genomic regions embedded in yeast artificial chromosomes (YACs). We have designed a yeast-bacteria shuttle vector, pClasper, that can be used to clone specific regions of interest from YACs by homologous recombination. The im...
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| Udgivet i: | Nucleic Acids Res |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
1995
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| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307474/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8532528/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.23.4850 |
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