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Initiation of unidirectional ColE2 DNA replication by a unique priming mechanism.
The ColE2 DNA can be replicated in an in vitro system consisting of a crude extract of Escherichia coli cells. DNA synthesis requires a plasmid-coded protein (Rep) and host DNA polymerase I but not host RNA polymerase. Replication starts at a fixed region containing the origin and proceeds unidirect...
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| Hauptverfasser: | , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1995
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307362/ https://ncbi.nlm.nih.gov/pubmed/7479084 |
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