Incorporation of a fluorescent guanosine analog into oligonucleotides and its application to a real time assay for the HIV-1 integrase 3'-processing reaction.
We have synthesized a highly fluorescent (quantum yield 0.88) guanosine analog, (3-methyl-8-(2-deoxy-beta-D-ribofuranosyl) isoxanthopterin (3-Mi) in a dimethoxytrityl, phosphoramidite protected form, which can be site-specifically inserted into oligonucleotides through a 3',5'-phosphodiester linkage...
Furkejuvvon:
| Publikašuvnnas: | Nucleic Acids Res |
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| Váldodahkkit: | , , , , |
| Materiálatiipa: | Artigo |
| Giella: | Inglês |
| Almmustuhtton: |
Oxford University Press
1995
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| Liŋkkat: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307124/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7659509/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.15.2872 |
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