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Gram-scale purification of phosphorothioate oligonucleotides using ion-exchange displacement chromatography.

The purification of oligonucleotides by ion-exchange displacement chromatography is demonstrated on the gram-scale. Using a 50 mmD x 100 mmL (203 ml) column operated in the displacement mode, 1.2 g of a 24mer phosphorothioate oligonucleotide was purified. Product yield for this separation was 70% (7...

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Bibliografski detalji
Glavni autori: Gerstner, J A, Pedroso, P, Morris, J, Bergot, B J
Format: Artigo
Jezik:Inglês
Izdano: 1995
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC307020/
https://ncbi.nlm.nih.gov/pubmed/7610058
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