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Gram-scale purification of phosphorothioate oligonucleotides using ion-exchange displacement chromatography.
The purification of oligonucleotides by ion-exchange displacement chromatography is demonstrated on the gram-scale. Using a 50 mmD x 100 mmL (203 ml) column operated in the displacement mode, 1.2 g of a 24mer phosphorothioate oligonucleotide was purified. Product yield for this separation was 70% (7...
Αποθηκεύτηκε σε:
| Κύριοι συγγραφείς: | , , , |
|---|---|
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
1995
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| Διαθέσιμο Online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307020/ https://ncbi.nlm.nih.gov/pubmed/7610058 |
| Ετικέτες: |
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