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Mammalian assay for site-specific DNA damage processing using the human H-ras proto-oncogene.
The human genomic H-ras proto-oncogene was inserted into an Epstein-Barr virus (EBV) vector (p220.2) that replicates synchronously with the cell cycle. Unique restriction enzyme sites, 30 bp apart, were created on either side of codon 12 to enable the construction of gapped heteroduplex (GHD) DNA. D...
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| Autori principali: | , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
1995
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| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307017/ https://ncbi.nlm.nih.gov/pubmed/7610055 |
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