Enhancement of retrovirus-mediated gene transduction efficiency by transient overexpression of the amphotropic receptor, GLVR-2.

Eitemau Tebyg

• Efficient Retrovirus‐mediated Cytokine‐gene Transduction of Primary‐cultured Human Glioma Cells for Tumor Vaccination Therapy
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• Efficient Transduction by an Amphotropic Retrovirus Vector Is Dependent on High-Level Expression of the Cell Surface Virus Receptor
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• The level of mRNA encoding the amphotropic retrovirus receptor in mouse and human hematopoietic stem cells is low and correlates with the efficiency of retrovirus transduction.
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• Poor transduction efficiency of human hematopoietic progenitor cells by a high-titer amphotropic retrovirus producer cell clone.
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• Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges.
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