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Transformation of Escherichia coli with large DNA molecules by electroporation.

We have examined bacterial electroporation with a specific interest in the transformation of large DNA, i.e. molecules > 100 kb. We have used DNA from bacterial artificial chromosomes (BACs) ranging from 7 to 240 kb, as well as BAC ligation mixes containing a range o different sized molecules. Th...

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Detalhes bibliográficos
Main Authors: Sheng, Y, Mancino, V, Birren, B
Formato: Artigo
Idioma:Inglês
Publicado em: 1995
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC306974/
https://ncbi.nlm.nih.gov/pubmed/7596828
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