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Transformation of Escherichia coli with large DNA molecules by electroporation.
We have examined bacterial electroporation with a specific interest in the transformation of large DNA, i.e. molecules > 100 kb. We have used DNA from bacterial artificial chromosomes (BACs) ranging from 7 to 240 kb, as well as BAC ligation mixes containing a range o different sized molecules. Th...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1995
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC306974/ https://ncbi.nlm.nih.gov/pubmed/7596828 |
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