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Speckled Microtubules Improve Tracking in Motor-Protein Gliding Assays

Gliding assays of motor proteins such as kinesin, dynein, and myosin are commonly carried out with fluorescently labeled microtubules or filamentous actin. In this paper, we show that speckled microtubules (MTs), prepared by copolymerizing 98% unlabeled tubulin with 2% rhodamine-labeled tubulin, can...

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Detalhes bibliográficos
Main Authors: Chisena, Ernest N., Wall, R. Andrew, Macosko, Jed C., Holzwarth, George
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3068918/
https://ncbi.nlm.nih.gov/pubmed/17406081
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1088/1478-3975/4/1/002
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