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Modified nucleotides reveal the indirect role of the central base pairs in stabilizing the lac repressor-operator complex.
Guanine residues in the lac operator were replaced by 2-aminopurine or purine analogues, pairing the modified nucleotides with C. The observed equilibrium dissociation constants for lac repressor binding to substituted operators were measured in 10 mM Tris, 150 mM KCl, 0.1 mM EDTA, 0.1 mM DTE, pH 7....
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1995
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC306889/ https://ncbi.nlm.nih.gov/pubmed/7784203 |
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