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Preparation of probe-modified RNA with 5-mercapto-UTP for analysis of protein-RNA interactions.
We report a modified synthesis for 5-mercapto-UTP (5-SH-UTP) and its use for analysis of protein-RNA interactions utilizing Escherichia coli and T7 RNA polymerases and yeast RNA polymerases I and III. 5-SH-UTP did not affect transcriptional pausing, Rho-independent termination or recognition of the...
Sparad:
| Huvudupphovsmän: | , , |
|---|---|
| Materialtyp: | Artigo |
| Språk: | Inglês |
| Publicerad: |
1995
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| Länkar: | https://ncbi.nlm.nih.gov/pmc/articles/PMC306836/ https://ncbi.nlm.nih.gov/pubmed/7537876 |
| Taggar: |
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