Optimization of Storage and Shipment of Cryopreserved Peripheral Blood Mononuclear Cells from HIV-Infected and Uninfected Individuals for ELISPOT Assays

Functional immunologic assays using cryopreserved peripheral blood mononuclear cells (PBMC) are influenced by blood processing, storage and shipment. The objective of this study was to compare the viability, recovery and ELISPOT results of PBMC stored and shipped in liquid nitrogen (LN/LN) or stored...

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Hauptverfasser: Weinberg, Adriana, Song, Lin Ye, Wilkening, Cindy L., Fenton, Terry, Hural, John, Louzao, Raul, Ferrari, Guido, Etter, Paige E., Berrong, Mark, Canniff, Jennifer D., Carter, Donald, De fawe, Olivier D., Garcia, Ambrosia, Garrelts, Tania L., Gelman, Rebecca, Lambrecht, Linda K., Pahwa, Savita, Pilakka-Kanthikeel, Sudheesh, Shugarts, David L., Tustin, Nancy B.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3068047/
https://ncbi.nlm.nih.gov/pubmed/20888337
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2010.09.032
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