Contribution of the Rgg Transcription Regulator to Metabolism and Virulence of Streptococcus suis Serotype 2

The Rgg-like regulators, a family of transcription factors commonly found in many Gram-positive bacteria, play multiple roles, especially in the control of pathogen virulence. Here, we report an rgg homologue from a Chinese isolate, 05ZYH33, of Streptococcus suis serotype 2 (SS2). Deletion of the rg...

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Main Authors: Zheng, Feng, Ji, Hongfeng, Cao, Min, Wang, Changjun, Feng, Youjun, Li, Ming, Pan, Xiuzhen, Wang, Jing, Qin, Yuehong, Hu, Fuquan, Tang, Jiaqi
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2011
Assuntos:
Molecular Pathogenesis
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3067506/
https://ncbi.nlm.nih.gov/pubmed/21149588
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.00193-10
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spelling pubmed-30675062011-09-01 Contribution of the Rgg Transcription Regulator to Metabolism and Virulence of Streptococcus suis Serotype 2 Zheng, Feng Ji, Hongfeng Cao, Min Wang, Changjun Feng, Youjun Li, Ming Pan, Xiuzhen Wang, Jing Qin, Yuehong Hu, Fuquan Tang, Jiaqi Infect Immun Molecular Pathogenesis The Rgg-like regulators, a family of transcription factors commonly found in many Gram-positive bacteria, play multiple roles, especially in the control of pathogen virulence. Here, we report an rgg homologue from a Chinese isolate, 05ZYH33, of Streptococcus suis serotype 2 (SS2). Deletion of the rgg gene in SS2 increased its adhesion to Hep-2 cells and hemolytic activity in vitro. Significantly, inactivation of the rgg gene attenuated SS2 virulence in an experimental piglet infection model. Using DNA microarrays and quantitative reverse transcription-PCR, we found that the Rgg regulator affects the transcriptional profile of 15.87% (n = 345) of all of the annotated chromosomal genes, including those involved in nonglucose carbohydrate metabolism, DNA recombination, protein biosynthesis, bacterial defense mechanisms, and others. It was experimentally verified that the deletion of rgg in SS2 reduced the utilization of nonglucose carbohydrates, such as lactose and maltose. In addition, the rgg gene was found to be associated with changes in the bacterial microscopic phenotype and growth curve. These data suggested that Rgg in SS2 is a global transcriptional regulator that plays an important role in promoting SS2 bacterial survival during pathogen-host interaction. American Society for Microbiology (ASM) 2011-03 2010-12-13 /pmc/articles/PMC3067506/ /pubmed/21149588 http://dx.doi.org/10.1128/IAI.00193-10 Text en Copyright © 2011, American Society for Microbiology
institution US NLM
collection PubMed Central
language Inglês
format Artigo
topic Molecular Pathogenesis
spellingShingle Molecular Pathogenesis
Zheng, Feng
Ji, Hongfeng
Cao, Min
Wang, Changjun
Feng, Youjun
Li, Ming
Pan, Xiuzhen
Wang, Jing
Qin, Yuehong
Hu, Fuquan
Tang, Jiaqi
Contribution of the Rgg Transcription Regulator to Metabolism and Virulence of Streptococcus suis Serotype 2
description The Rgg-like regulators, a family of transcription factors commonly found in many Gram-positive bacteria, play multiple roles, especially in the control of pathogen virulence. Here, we report an rgg homologue from a Chinese isolate, 05ZYH33, of Streptococcus suis serotype 2 (SS2). Deletion of the rgg gene in SS2 increased its adhesion to Hep-2 cells and hemolytic activity in vitro. Significantly, inactivation of the rgg gene attenuated SS2 virulence in an experimental piglet infection model. Using DNA microarrays and quantitative reverse transcription-PCR, we found that the Rgg regulator affects the transcriptional profile of 15.87% (n = 345) of all of the annotated chromosomal genes, including those involved in nonglucose carbohydrate metabolism, DNA recombination, protein biosynthesis, bacterial defense mechanisms, and others. It was experimentally verified that the deletion of rgg in SS2 reduced the utilization of nonglucose carbohydrates, such as lactose and maltose. In addition, the rgg gene was found to be associated with changes in the bacterial microscopic phenotype and growth curve. These data suggested that Rgg in SS2 is a global transcriptional regulator that plays an important role in promoting SS2 bacterial survival during pathogen-host interaction.
author Zheng, Feng
Ji, Hongfeng
Cao, Min
Wang, Changjun
Feng, Youjun
Li, Ming
Pan, Xiuzhen
Wang, Jing
Qin, Yuehong
Hu, Fuquan
Tang, Jiaqi
author_facet Zheng, Feng
Ji, Hongfeng
Cao, Min
Wang, Changjun
Feng, Youjun
Li, Ming
Pan, Xiuzhen
Wang, Jing
Qin, Yuehong
Hu, Fuquan
Tang, Jiaqi
author_sort Zheng, Feng
title Contribution of the Rgg Transcription Regulator to Metabolism and Virulence of Streptococcus suis Serotype 2
title_short Contribution of the Rgg Transcription Regulator to Metabolism and Virulence of Streptococcus suis Serotype 2
title_full Contribution of the Rgg Transcription Regulator to Metabolism and Virulence of Streptococcus suis Serotype 2
title_fullStr Contribution of the Rgg Transcription Regulator to Metabolism and Virulence of Streptococcus suis Serotype 2
title_full_unstemmed Contribution of the Rgg Transcription Regulator to Metabolism and Virulence of Streptococcus suis Serotype 2
title_sort contribution of the rgg transcription regulator to metabolism and virulence of streptococcus suis serotype 2
publisher American Society for Microbiology (ASM)
publishDate 2011
url https://ncbi.nlm.nih.gov/pmc/articles/PMC3067506/
https://ncbi.nlm.nih.gov/pubmed/21149588
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.00193-10
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