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Shuttle Vector System for Methanococcus maripaludis with Improved Transformation Efficiency

We have identified an open reading frame and DNA element that are sufficient to maintain shuttle vectors in Methanococcus maripaludis. Strain S0001, containing ORF1 from pURB500 integrated into the M. maripaludis genome, supports a significantly smaller shuttle vector, pAW42, and a 7,000-fold increa...

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Detalhes bibliográficos
Main Authors: Walters, Alison D., Smith, Sarah E., Chong, James P. J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3067461/
https://ncbi.nlm.nih.gov/pubmed/21296937
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02919-10
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