Natural Competence in the Hyperthermophilic Archaeon Pyrococcus furiosus Facilitates Genetic Manipulation: Construction of Markerless Deletions of Genes Encoding the Two Cytoplasmic Hydrogenases

In attempts to develop a method of introducing DNA into Pyrococcus furiosus, we discovered a variant within the wild-type population that is naturally and efficiently competent for DNA uptake. A pyrF gene deletion mutant was constructed in the genome, and the combined transformation and recombinatio...

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Detalhes bibliográficos
Main Authors: Lipscomb, Gina L., Stirrett, Karen, Schut, Gerrit J., Yang, Fei, Jenney, Francis E., Scott, Robert A., Adams, Michael W. W., Westpheling, Janet
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2011
Assuntos:
Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3067412/
https://ncbi.nlm.nih.gov/pubmed/21317259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02624-10
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