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The construction and analysis of M13 libraries prepared from YAC DNA.
Yeast artificial chromosomes (YACs) provide a powerful way to isolate and map large regions of genomic DNA and their use in genome analysis is now extensive. We modified a series of procedures to produce high quality shotgun libraries from small amounts of YAC DNA. Clones from several different libr...
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Main Authors: | , , , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
1995
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Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC306736/ https://ncbi.nlm.nih.gov/pubmed/7899089 |
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