An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching

Cyan fluorescent proteins (CFPs), such as Cerulean, are widely used as donor fluorophores in Förster resonance energy transfer (FRET) experiments. Nonetheless, the most widely used variants suffer from drawbacks that include low quantum yields and unstable flurorescence. To improve the fluorescence...

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Detalhes bibliográficos
Main Authors: Markwardt, Michele L., Kremers, Gert-Jan, Kraft, Catherine A., Ray, Krishanu, Cranfill, Paula J. C., Wilson, Korey A., Day, Richard N., Wachter, Rebekka M., Davidson, Michael W., Rizzo, Megan A.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2011
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3066204/
https://ncbi.nlm.nih.gov/pubmed/21479270
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0017896
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