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An Improved Cerulean Fluorescent Protein with Enhanced Brightness and Reduced Reversible Photoswitching

Cyan fluorescent proteins (CFPs), such as Cerulean, are widely used as donor fluorophores in Förster resonance energy transfer (FRET) experiments. Nonetheless, the most widely used variants suffer from drawbacks that include low quantum yields and unstable flurorescence. To improve the fluorescence...

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Bibliografiske detaljer
Main Authors: Markwardt, Michele L., Kremers, Gert-Jan, Kraft, Catherine A., Ray, Krishanu, Cranfill, Paula J. C., Wilson, Korey A., Day, Richard N., Wachter, Rebekka M., Davidson, Michael W., Rizzo, Megan A.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3066204/
https://ncbi.nlm.nih.gov/pubmed/21479270
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0017896
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