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Analysis of protein kinase A activity in insulin-secreting cells using a cell-penetrating protein substrate and capillary electrophoresis

A cell-penetrating, fluorescent protein substrate was developed to monitor intracellular protein kinase A (PKA) activity in cells without the need for cellular transfection. The PKA substrate (PKAS) was prepared with a 6×histidine purification tag, an enhanced green fluorescent protein (EGFP) report...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Rauf, Femina, Huang, Yiding, Muhandiramlage, Thusitha P., Aspinwall, Craig A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3064950/
https://ncbi.nlm.nih.gov/pubmed/20458471
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00216-010-3776-7
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