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DNA denatures upon drying after ethanol precipitation.

We have observed that ethanol precipitation and subsequent drying of small (less than 400 bp) radiolabelled DNA fragments is able to induce a transition to a form that migrates aberrantly on acrylamide gels. This unusual form has increased sensitivity to S1 nuclease, decreased sensitivity to restric...

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Detalhes bibliográficos
Main Authors: Svaren, J, Inagami, S, Lovegren, E, Chalkley, R
Formato: Artigo
Idioma:Inglês
Publicado em: 1987
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC306402/
https://ncbi.nlm.nih.gov/pubmed/3684571
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