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Cleavage of methylated CCCGGG sequences containing either N4-methylcytosine or 5-methylcytosine with MspI, HpaII, SmaI, XmaI and Cfr9I restriction endonucleases.

The cleavage specificity of R.Cfr9I was determined to be C decreases CCGGG whereas the methylation specificity of M.Cfr9I was C4mCCGGG. The action of MspI, HpaII, SmaI, XmaI and Cfr9I restriction endonucleases on an unmethylated parent d(GGACCCGGGTCC) dodecanucleotide duplex and a set of oligonucleo...

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Autors principals: Butkus, V, Petrauskiene, L, Maneliene, Z, Klimasauskas, S, Laucys, V, Janulaitis, A
Format: Artigo
Idioma:Inglês
Publicat: 1987
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC306195/
https://ncbi.nlm.nih.gov/pubmed/2821492
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