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One-Sample Approach to Determining the Relative Orientations of Proteins in Ternary and Binary Complexes from Residual Dipolar Coupling Measurements

We present a procedure that supports the acquisition of (1)H-(15)N residual dipolar coupling (RDC) values for individual subunits in binary or ternary protein assemblies from a single experimental sample. Our method relies on asymmetric labeling of each subunit with the following scheme: species A u...

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Detalhes bibliográficos
Main Authors: Tonelli, Marco, Masterson, Larry R., Cornilescu, Gabriel, Markley, John L., Veglia, Gianluigi
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3061545/
https://ncbi.nlm.nih.gov/pubmed/19764746
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja904766g
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