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One-Sample Approach to Determining the Relative Orientations of Proteins in Ternary and Binary Complexes from Residual Dipolar Coupling Measurements
We present a procedure that supports the acquisition of (1)H-(15)N residual dipolar coupling (RDC) values for individual subunits in binary or ternary protein assemblies from a single experimental sample. Our method relies on asymmetric labeling of each subunit with the following scheme: species A u...
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Main Authors: | , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2009
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3061545/ https://ncbi.nlm.nih.gov/pubmed/19764746 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja904766g |
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