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A simple and rapid preparation of M13 sequencing templates for manual and automated dideoxy sequencing.
A simple and rapid procedure for the preparation of M13 single stranded DNA sequencing templates which does not involve phenol extractions and alcohol precipitations is described. Bacteriophages are precipitated from media supernatants with acetic acid and recovered on glass fiber filters. Subsequen...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1987
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| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC306003/ https://ncbi.nlm.nih.gov/pubmed/3615197 |
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